Originally Posted by: TexAxe Also, check the saddle where the string crosses over it. There may be a groove or sharp edge preventing your string from intonating correctly. If all else fails, take it to a good luthier/repair person. They can probably fix fairly easily and cheaply.

I forgot one thing. TexAxe is correct. If the saddle has any imperfections in manufacture, this could cause intonation problems as well. It's a lot of work but one way to prove that is to change the saddle in question with another one known to work properly. This is a lot of work and if you feel comfortable with it, fine. If not, best do as TexAxe has suggested--take it to a reputable luthier.

Best wishes,

Bobby Howe