Aaron - yes! As you practice, you will find that your fingers figure out where they have to go. Once you get your reference point, off you go!

Does your guitar have dots on the side of the neck that you can see above? These would be the same as the dots on the fretboard. If they are there, use them as your guide when standing.

Sitting down you may be turning the guitar a bit so you can see easier. Standing up you can't do this at all - so it's good practice to help with proper form.

I find I have to look down more for my strumming hand rather than my fretting hand. Just by playing I've found that my strumming hand has gotten much more accurate and that I can hit any string I want when I need to.

Good luck.