Originally Posted by: Dr_simonIf it is any conciliation I am having a bad day also !

I have spent months trying to isolate a mutant yeast allele that causes a phenotype that I can see.

I finally got back a plasmid only to discover that it has no mutations in it.

This means that I either have not rescued the right allele or the phenotype is due to a mutation in a gene I don't care about.

Both are bad !

bugger !

Well, the identity of one known allele does not always enable prediction of
the phenotype, how do you know which one to isolate?
Anyway, that does sounds like a bad day to me.
"Oh spare me the arbitrariness of science!" Polhem once said.