Originally Posted by: Dr_simonIf it is any conciliation I am having a bad day also !
I have spent months trying to isolate a mutant yeast allele that causes a phenotype that I can see.
I finally got back a plasmid only to discover that it has no mutations in it.
This means that I either have not rescued the right allele or the phenotype is due to a mutation in a gene I don't care about.
Both are bad !
bugger !
Yeah, I hate when that happens!!! :mad: :mad: :mad:
... and that's all I have to say about that.
[U]ALL[/U] generalizations are [U]WRONG[/U]
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[U]ALL[/U] generalizations are [U]WRONG[/U]
[/sarcasm]