Originally Posted by: natashagranger

My question is, what is my reference point for the low E? If my low E is out of tune, how would I be able to know without checking my electronic tuner?

You either have to use an external reference (a tuner, or other instrument that you know is in tune) or you have to have perfect pitch or pitch recall.

It's possible to just tune the guitar to itself making sure all the strings are tuned relative to each other. But there is no guarantee that it is in absolute pitch without an external reference.